Untargeted Metabolomics Service

Home
Metabolomics Service
Untargeted Metabolomics Service

Online Inquiry

v-innovate Technologies committed to providing reliable and cost-effective untargeted metabolomics services for academia and various industries. Our solutions are unbiased and detect as many small molecule metabolites as possible in a variety of samples (such as cells, tissues, body fluids, plants, microorganisms, food, etc.), and meet your needs.

Overview

Untargeted metabolomics services are mainly for comprehensive biochemical information of metabolism in organisms. Based on liquid chromatography-mass spectrometry technology (LC-MS), qualitative and quantitative analysis of almost all metabolites under certain physiological conditions or under specific conditions are carried out to find and identify different metabolites. Our untargeted metabolome analysis platform can accurately and rapidly analyze the small molecule metabolites covering more than 95% pathway in various biological samples (blood, urine, tissue, saliva, amniotic fluid, cells and cell fluid, etc.) and plant samples. Untargeted metabolomics, to the greatest extent, reflects the multiple dynamic responses of living organisms to external stimuli, pathophysiological changes and their own gene mutations in metabolite levels in vivo, providing a new perspective for disease diagnosis, pathological research, new drug development, drug toxicology and other studies.

Our technology platform can serve multiple industries, including biomedicine, pharmaceuticals, agriculture, animal husbandry, nutrition and other fields.

What we can provide:

Metabolic profile analysis
Screening for differential metabolites
Metabolic pathway analysis of differential metabolites
Discover new biomarkers

Instrumentation

v-innovate Technologies uses an advanced LC-MS/MS system for untargeted metabolomics analysis services.

UHPLC-QTOF-MS (Agilent 1290)
UHPLC + AB QTOF 5600(ACQUITY UHPLC)

Workflow for untargeted metabolomics service

Sample Preparation

Animal tissues: ≥ 200 mg
Cell samples: Collect cell pellets, rinse with PBS 3 times to remove residual serum. The sample volume is 1×107 or the cell pellet is not less than 50 μl.
Cellular supernatant: ≥ 1mL
Plasma/serum sample: fresh blood is taken out, anticoagulated or coagulated, centrifuged at 4°C, and the supernatant is taken to obtain plasma/serum. During the preparation process, ensure that hemolysis is not allowed, and the recommended sample volume should not be less than 250 μl.
Urine: ≥ 1ml
Saliva: ≥ 0.5ml
Follicular fluid, cerebrospinal fluid, lymphatic fluid, etc.: ≥ 200 μL
Viruses, bacteria or fungi: wet precipitate weight ≥ 200 mg
Stool and intestinal contents ≥ 200 mg
Plant tissues: ≥200 mg
Plant seeds: ≥100 mg

Please contact us for other special samples.

Data Analysis of Untargeted Metabolomics

Quality control: QC sample results including TIC and PCA results
Quantification and characterization results of differential metabolites
Univariate analysis: T test and/or ANOVA analysis of all identified metabolites
Multivariate analysis: PCA, PLS-DA, OPLS-DA of all identified metabolites
Functional analysis: Differential metabolite cluster analysis; Differential metabolite KEGG and KEGG enrichment analysis; Metabolite difference correlation analysis
Biomarker ROC analysis

v-innovate Technologies offers several approaches to provide untargeted metabolomics service and deliver precise and detailed data and analysis report. We can also customize methods or establish new methods together with our collaborators to meet the specific needs of any project. If you have any questions, you can tell us through the inquiry form, and our technicians will communicate with you.