Tear Metabolomics Service

Online Inquiry

Based on liquid chromatography-mass spectrometry (LC-MS) technology, v-innovate Technologies provides fast and accurate metabolomics analysis of tear samples to meet your different needs.

Overview

Tears not only moisten the eyeballs and produce immunity and defense, but also contain a lot of molecular information related to human physiology and pathology. The ingestion of therapeutic drugs and drugs of abuse can also be excreted through lacrimal secretion. In addition, because the eyes are directly exposed to the air, volatiles, aerosols and soluble particles in the air may also enter the eyes and be transferred to the tears. Due to the small secretion volume, complex composition, high salinity, and low metabolite concentration of tear fluid, the collection and analysis of trace tear fluid is extremely challenging.

According to different analysis needs, v-innovate Technologies provides LC-MS-based analysis services for tear samples to help you analyze the target metabolites or the full spectrum of metabolism in the sample.

What We Can Provide:

Untargeted metabolomics analysis:

Metabolic profile analysis
Screening for differential metabolites
Metabolic pathway analysis of differential metabolites
Discover new biomarkers

Fig 1. The workflow of untargeted metabolomics analysis

Targeted metabolomics analysis:

Mode: SRM / MRM
Analysis content: Standard curve creation. Raw data preprocessing. Absolute quantification of metabolites.

Fig 2. The workflow of target metabolomics analysis

Sample Requirements

The collection of human tears involves strict safety requirements. Please contact our technical staff for specific requirements. Quick-freeze in liquid nitrogen and store at -80°C, and send it on dry ice.

Deliverables

Experimental procedure
Parameters of liquid chromatography / gas chromatography and MS
MS raw data files and MS data quality checks
Bioinformatics analysis report

Turnaround time: 1-4 weeks

Bioinformatics Analysis

Standard analysis	1. Data quality control (QC correlation analysis, PCA analysis of total samples)
	2. Qualitative and quantitative results of metabolites
	3. Differential metabolite screening (PCA, PLS-DA dimensionality reduction treatment, volcano map)
	4. Differential metabolite analysis: Venn diagram, cluster heat map, metabolite correlation analysis, Z-score analysis, pathway analysis, ROC analysis
Personalized analysis	Weighted gene co-expression network analysis (WGCNA)
Personalized analysis	One way ANOVA

v-innovate Technologies provides metabolomics services for tear samples, and will deliver accurate and detailed data and analysis reports to you. If you want to detect other metabolites but have not found them, you can tell us through the inquiry form, and our technicians will communicate with you.