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Wheat germ agglutinin (WGA) is one of the most widely used lectins, isolated from the germ of common wheat.
WGA has two subunits and its molecular weight is 36 kDa.
WGA protects wheat from insects, yeast and bacteria.
The acceptor sugar of WGA is N-acetylglucosamine, which preferentially binds to dimers and trimers of this sugar.
WGA lectin agglutinates red blood cells and most types of malignant cells.
Schematic diagram of WGA structure
Sample demand |
Plant root / stem / leaf / fruit / seed > 200 mg fresh weight, freeze-dried sample > 0.5 g Animal tissue / feces related samples > 200mg / sample Cells / bacteria > 107 Serum / plasma / urine / ruminal fluid / cerebrospinal fluid / amniotic fluid and other homogeneous liquid samples > 200 µl. (Hemolysis should be avoided. Cerebrospinal fluid can be as low as 100 µl.) For other sample types, please consult technical support or sales. |
Sample mixed and repeat |
In order to ensure the accuracy of the samples and reduce systematic errors during sampling, it is necessary to select more than 3 materials with the same condition for each sample. (※ The same condition refers to the same period, basically the same phenotype, and the same part.) It is recommended that prepare more than 6 biological replicates. |
Storage and transportation |
Quick-frozen preservation with liquid nitrogen can minimize the leakage time of plant samples at room temperature, and store it at -80℃. Dry ice transportation (about 3~4kg dry ice is consumed every day, please use sufficient dry ice for transportation). |