Thapsigargin Analysis Service

v-innovate Technologies' targeted metabolomics services target specific metabolites and pathways of interest, which can accurately monitor dynamic metabolic processes, reveal related metabolic mechanisms and verify potential metabolic biomarkers, to guide your research with reliable and accurate measurement results. CPR is based on LC-MS/GC-MS technology, using target compound standards for method optimization, method validation, biological sample collection, metabolite extraction, mass spectrometry detection, data analysis, etc.

About Thapsigargin

Thapsigargin is extracted from thapsiagarganica.

Thalassene is classified as guaiacolactone.

It acts as an effective, cell-permeable, IP3-independent intracellular calcium release agent, and can prevent the transient increase of intracellular Ca²⁺ induced by angiostatin and endostatin.

It induces apoptosis by destroying the level of free Ca²⁺ in the cell.

The molecular formula of thapsigargin is C34H50O12, and the CAS number is 67526-95-8.

MS-based technical means can realize the qualitative and quantitative analysis of thapsigargin.

Structure of thapsigargin

Detection Platform

• LC-MS/MS

Waters ACQUITY UPLC, MS-SCIEX QTRAP 4500/5500/6500

• GC-MS

Agilent 7890B-5977A, Thermo TRACE 1300-TSQ 9000

Technical Workflow of Targeted Metabolomics of Thapsigargin

Analysis Content

• Standard curve creation
• Raw data preprocessing
• Absolute quantitation of thapsigargin

Sample Preparation Suggestions

※ There is no restriction on sample varieties. For varieties with special requirements or rare varieties, please contact us for more information. After collecting the samples, they were quickly frozen in liquid nitrogen, and then transferred to -80°C for storage. Transported on dry ice.

Feedback to Customers

• A detailed technical report will be provided at the end of the entire project, including experimental procedures and instrument parameters.
• The report also includes quantitative results of targeted metabolites, such as name, abbreviation, molecular formula, molecular weight and CAS number of the analyte.
• Part of the mass spectrum picture
• Raw data

Project Cycle

• A standard experimental procedure takes about 1 ~ 4 weeks.

v-innovate Technologies has an integrated separation, detection, identification and quantification system, with excellent robustness and reproducibility, high sensitivity and ultra-sensitivity, and can provide fast and reliable targeted metabolomics services. Our one-stop service model can help you achieve a seamless connection between upstream and downstream experiments. Coupled with personalized analysis services, your analysis can reach the final research purpose. If you have any questions or specific requirements, please feel free to contact us.