Synthesis and Degradation of Ketone Bodies Analysis Service

v-innovate Technologies is a pioneer in metabolism service and provides reliable, professional and cost-effective synthesis and degradation of ketone bodies analysis service in animal. We leverage our rich experience, professional expertise, and advanced technologies to speed up your scientific purposes of synthesis and degradation of ketone bodies analysis in animal.

Overview

Ketone bodies refer to water-soluble molecules including acetoacetate, beta-hydroxybutyrate and acetone, which are produced as by-products when fatty acids are broken down for energy in the liver and kidney. Ketone body metabolism includes ketone body synthesis (ketogenesis) and degradation (ketolysis). Ketogenesis is the biochemical process through which organisms produce ketone bodies through breakdown of fatty acids and ketogenic amino acids. This process provides energy under circumstances such as fasting or caloric restriction to certain organs like the brain and heart. In contrast, ketosis is a general response to low glucose availability, which provides an extra energy source for the brain in the form of ketones. Therefore, either excessive or lack of ketone cause pathological conditions like diabetes and hypoglycemia. As a high-throughput tool, synthesis and degradation of ketone bodies analysis provides a powerful way to diagnosis and gain new insights in disease mechanism or treatment. In v-innovate Technologies, we provide reliable, rapid and cost-effective synthesis and degradation of ketone bodies analysis related service to speed up your research aims ranging from diseases diagnosis to gaining new insights in disease mechanism or treatment.

The Main Applications of Synthesis and Degradation of Ketone Bodies Service

Identifying  novel biomarkers of diseases

Improving diagnose diseases

Accelerating development of novel drugs

Gaining new mechanism of diseases

Advantages of Our Synthesis and Degradation of Ketone Bodies Service

Constantly optimized protocol and analytical software

Professional experiment design

Quick turnaround time

High accuracy, specificity, and sensitivity

Service Workflow

v-innovate Technologies provides synthesis and degradation of ketone bodies analysis service in a reliable and effective manner, based on our cutting-edge high-performance liquid chromatography (HPLC) platforms. The experimental procedures contain four main procedures: sample collection, metabolites extraction, HPLC data analysis and bioinformatics analysis (Figure 1). Our service will be tailored to specific samples and needs for optimal results.

Figure 1. The overall workflow of synthesis and degradation of ketone bodies

The Potential Detectable Synthesis and Degradation of Ketone Bodies Related Metabolites at v-innovate Technologies

Acetyl-CoA	Acetoacetyl-CoA	β-Hydroxy β-methylglutaryl-CoA
Acetoacetate	3-Hydroxybutyrate	Ketone

Sample Requirements

We can analyze a wide range of biological materials including but not limited to cells and solid tissues from humans and animals, such as mice, rats, rabbits. If you need transport your samples to us, please follow the following requirements for different types of sample:

• Blood/plasma: 500ul/sample
• Urine: 1ml/sample
• Tissue: 200mg/sample
• Cells: 1x10⁷/sample
• Feces: 500mg/sample
• Shipment condition: dry ice

Report Delivery

• Experimental protocols
• Instrumental factors of HPLC
• The raw data files of HPLC and the summary of HPLC data quality
• Bioinformatics analysis report

Based on advanced HPLC platforms for the determination of synthesis and degradation of ketone bodies professional bioinformatic analysis software and experienced technicians and scientists, v-innovate Technologies provides customer-tailored synthesis and degradation of ketone bodies analysis service with rapid experimental procedures and easy to read report, to accelerate your scientific research.

References

1. Laffel L. Ketone bodies: a review of physiology, pathophysiology and application of monitoring to diabetes. Diabetes Metab Res Rev.1999.15:412-426.
2. Sass Jörn Oliver. Inborn errors of ketogenesis and ketone body utilization. J Inherit Metab Dis. 2012.35:23-28.