Strigolactone (SL) Analysis Service

Applications of SL Analysis

• Elucidate mechanisms of SL biosynthesis
• Clarify SL perception and signal transduction
• Reveal downstream physiological and phenotypic effects of SLs
• Sustainable agriculture

Advantages of Our SL Analysis Service

Improved sample preprocessing ensures high recovery rates and effectiveness

Qualitative and quantitative chemical analysis of 5-DS

Customizable protocols and data analyses

End-to-end, high-resolution SL analysis with comparable price

Service Workflow

We utilize the most advanced separation method, ultra-high performance liquid chromatography (UHPLC) coupled with mass spectrometry (MS) for SL analysis, to offer great separation efficiencies, sensitive detection, and short analysis time. LC-MS/MS in multiple-reaction monitoring (MRM) is employed for data acquisition. Our team of experts is experienced in handling a variety of samples, and our sample preprocessing method is proved to be safe, environmentally friendly, effective, and ensures consistently high recovery rates and maximum homogeneity between samples.

Quantification method: external reference method

Mode: MRM, capable of simultaneously detecting more than 1000 MRM ion pairs

Precision: ≤10^-9 g

Positive/Negative polarity switching time: ≤ 20 ms, allowing for the acquisition of Q1/Q3 MRM transition mass spectra in both ionization modes from a single LC-MS/MS run.

Analysis content:

• Standard curve creation
• Raw data preprocessing
• Absolute quantification of SLs
• Differential metabolites screening
• Optimal analyses such as KEGG pathway analysis and hierarchical clustering

List of Detectable SLs at v-innovate Technologies

Sample Requirements

Root exudates or tissue: >2 g. Provide fresh samples for best results. Samples should be frozen in liquid nitrogen immediately after collection, and then transferred to -80°C for storage.

At least 3 biological replicates.

Deliverable

• Experimental procedure
• Parameters of liquid chromatography and MS
• Extracted mitochondria and purified protein sample
• Purity analysis report
• MS raw data files and MS data quality checks
• 5-DS quantification data
• Custom data analysis report

v-innovate Technologies provides numerous plant hormone analyses based on state-of-the-art GC-MS/MS or LC-MS/MS systems. We are committed to clarifying the biosynthesis, signaling, action, and perception of a wide range of phytohormones.

References

1. Halouzka R, Zeljković S Ć, Klejdus B, et al. Analytical methods in strigolactone research. Plant Methods, 2020, 16: 1-13.
2. Wu H, Li H, Chen H, et al. Identification and expression analysis of strigolactone biosynthetic and signaling genes reveal strigolactones are involved in fruit development of the woodland strawberry (Fragaria vesca). BMC plant biology, 2019, 19(1): 1-19.
3. Seto Y, Yasui R, Kameoka H, et al. Strigolactone perception and deactivation by a hydrolase receptor DWARF14. Nature communications, 2019, 10(1): 1-10.
4. Aliche E B, Screpanti C, De Mesmaeker A, et al. Science and application of strigolactones. New Phytologist, 2020, 227(4): 1001-1011.