v-innovate Technologies' targeted metabolomics services target specific metabolites and pathways of interest, which can accurately monitor dynamic metabolic processes, reveal related metabolic mechanisms and verify potential metabolic biomarkers, to guide your research with reliable and accurate measurement results.
Sinalbin is a glucosinolate, found in the seeds of white mustard, Sinapis alba and many wild plant species.
Sinalbin is metabolized by myrosinase to form mustard oil (4-hydroxybenzyl isothiocyanate).
Its molecular formula is C14H19NO10S2, and its CAS number is 19253-84-0.
MS-based technical means can realize the qualitative and quantitative analysis of sinalbin (P-hydroxybenzyl glucosinolate).
Structure of sinalbin (P-hydroxybenzyl glucosinolate)
Waters ACQUITY UPLC, MS-SCIEX QTRAP 4500/5500/6500 and Waters Xevo TQ-S
Agilent 7890A-5975C, Agilent 7890B-5977A, Thermo TRACE 1300-TSQ 9000
Extract metabolites, precipitate proteins, etc.
Optimize the corresponding parameters, establish the chromatographic and mass spectrometry analysis conditions of the tested substance, and create the MRM analysis method.
Configure a series of different concentration standards and make a standard curve.
According to the results of the sample detection, combined with the standard curve, the statistical analysis of the substance content in the sample is carried out.
Sample type | Suggested sample quantity (per case) | Number of repetitions (recommended) |
---|---|---|
Plant tissue | ≥ 400 mg | Plant samples ≥ 3 cases / group Clinical samples ≥ 30 cases / group Animal samples ≥ 10 cases / group Cell samples ≥ 6 cases / group |
Plant seed | ≥ 200 mg | |
Blood (serum, plasma) | ≥ 200 μl | |
Urine | ≥ 200 μl | |
Animal tissue | ≥100 mg | |
Cell | 107 | |
Cell supernatant | ≥ 1ml | |
Bacteria or fungi | Wet weight of sediment ≥ 200 mg | |
Fermentation broth | ≥ 500 μl | |
Other special samples | Please contact us for consultation |
v-innovate Technologies will provide you with detailed technical reports, including