Short Chain Fatty Acids Analysis

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Short Chain Fatty Acids Analysis

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Based on gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) technologies, v-innovate Technologies provides fast and accurate qualitative and quantitative analysis of a variety of short-chain fatty acids (SCFAs) to meet your different needs.

Overview

SCFAs are mainly produced by the digestion of indigestible sugars such as dietary fiber, resistant starch, oligosaccharides, etc. in the colon by the fermentation of beneficial bacteria such as lactic acid bacteria and bifidobacteria.

SCFAs are gradually becoming known as signal molecules that regulate diet, intestinal microbes and the host. SCFAs can maintain the electrolyte balance in the intestine, induce cancer cell differentiation and apoptosis, and regulate gene expression. It can improve body composition, glucose homeostasis, blood lipid profile, reduce weight and colon cancer risk. As the main energy substrate, SCFAs can also have anti-inflammatory and anti-cancer effects. It also affects chronic diseases through various mechanisms, and plays an important role in maintaining the metabolic homeostasis of colonic epithelial cells, which can protect the colon from external damage and have a potentially positive impact on colon diseases.

Potential gut–brain pathways through which sCFas might modulate brain function (Boushra et al., 2019)

Based on GC-MS and LC-MS technologies, we developed an analysis platform for SCFAs analysis. The external standard method is used to quantify the SCFAs, and the internal standard is used to calibrate the SCFAs, which can achieve the absolute quantity. Our short-chain fatty acid analysis services can be applied to many industries, including biomedicine, pharmaceuticals, agriculture, animal husbandry, food, nutrition and other fields.

Service Workflow

To detect more than 15 short chain fatty acids, we follow the basic process shown below. At the same time, we will adjust the experimental method according to different natures of samples, since v-innovate Technologies has developed multiple novel sample preparation techniques.

Figure 1. Short chain fatty acids analysis service workflow.

Mode: MRM

Precision: pmol

Linear: R2 > 0.99

Analysis content:

Standard curve creation
Raw data preprocessing
Absolute quantification of short chain fatty acids

List of Detectable Short Chain Fatty Acids at v-innovate Technologies

Acetic acid Propionic acid Butyric acid Isobutyric acid Valeric acid Isovaleric acid Hexanoic acid Ethylmethylacetic acid Isocaproic acid 3-Methylpentanoic acid 2-Methylvaleric acid 3-Hydroxyisovaleric acid Lactic acid Malonic acid Succinic acid Glutaric acid

Advantages of Our Short Chain Fatty Acids Analysis Service

Rich experience, can handle a variety of samples
Developed advanced analysis platform based on GC-MS and LC-MS technology
Fast turnaround time, about 1~3 weeks
Provide flexible statistical analysis and bioinformatics analysis

Sample Requirements

Serum / plasma ≥ 100 ul/sample
Tissue: ≥ 50 mg/sample
Saliva: ≥ 100 mg/sample
Fresh feces: ≥ 50 mg/sample
Freeze-dried feces: ≥ 5 mg/sample
Cell: ≥ 1×10⁷
Intestinal contents ≥ 100mg/sample

Quick-freeze in liquid nitrogen and store at -80°C, and send it on dry ice.

Deliverables

Experimental procedure
Parameters of liquid chromatography / gas chromatography and MS
MS raw data files and MS data quality checks
Short chain fatty acids quantification data
Custom analysis report

v-innovate Technologies offers several approaches to provide short-chain fatty acids analysis service and deliver precise and detailed data and analysis report. If you want to detect other metabolites but have not found them, you can tell us through the inquiry form, and our technicians will communicate with you.

Reference

Dalile, B., Van Oudenhove, L., et al. (2019). The role of short-chain fatty acids in microbiota–gut–brain communication. Nature reviews Gastroenterology & hepatology, 16(8), 461-478.

For Research Use Only. Not for use in diagnostic procedures.

Short Chain Fatty Acids Analysis