Riboflavin Analysis Service

Riboflavin (Vitamin B2) is a major component of the cofactor of flavinase, which is involved in the complex biological oxidation process of the organism and has important pharmacological effects. In plant research, riboflavin also has a regulatory role in plant growth and disease resistance and defense. v-innovate Technologies is able to provide you with reliable, professional and affordable qualitative and quantitative riboflavin analysis services. Take advantage of our extensive experience, professional expertise and advanced technology to accelerate the progress of your project.

Overview

Riboflavin a water-soluble vitamin, is an essential nutrient in vertebrates, hence adequate dietary intake is necessary. Endogenous synthesis of riboflavin by microflora in the large intestine might contribute to a lesser extent. Riboflavin has a role in various metabolic pathways, serving mainly as an integral component of its crucial biologically active forms (flavocoenzymes flavin adenine dinucleotide and flavin mononucleotide). These flavocoenzymes ensure the functionality of tremendous flavoproteins including oxidases, dehydrogenases, monooxygenases and reductases, which play crucial roles in a variety of cellular function including mitochondrial electron transport chain, β-oxidation of fatty acids, redox homeostasis, citric acid cycle, apoptosis and more. Riboflavin is associated with the treatment of cancer, heart disease, high blood pressure, burns, arthritis and other diseases.

In plant research, riboflavin induces protein kinase gene expression and disease resistance and initiates molecular responses in plant growth signaling pathways.

v-innovate Technologies utilizes a UPLC-MS technology platform to enable riboflavin determination in different samples.

The Main Applications of Riboflavin Analysis Service

• Identifying novel biomarkers of diseases
• Improving diagnose diseases
• Accelerating development of novel drugs
• Gaining new mechanism of diseases

Advantages of Our Riboflavin Analysis Service

• Constantly optimized protocol and analytical software
• Professional experiment design
• Quick turnaround time
• High accuracy, specificity, and sensitivity

Service Workflow

List of Partial Detectable Riboflavin Related Metabolites at v-innovate Technologies

Sample Requirements

• Plant tissues: 400mg/sample
• Animal tissues: 200mg/sample
• Blood/plasma: 500ul/sample
• Urine: 1ml/sample
• Cells: 1x10⁷/sample
• Feces: 500mg/sample

Shipment condition: dry ice

Report Delivery

• Experimental procedures
• The parameters of liquid chromatograph and mass spectrometer
• HPLC and MS raw data files
• Metabolite quantitative data

References

1. Barile Maria, Giancaspero Teresa Anna, Leone Piero. et al. Riboflavin transport and metabolism in humans. J Inherit Metab Dis. 2016.39:545-557.
2. Mosegaard Signe, Dipace Graziana, Bross Peter. et al. Riboflavin Deficiency-Implications for General Human Health and Inborn Errors of Metabolism. Int J Mol Sci. 2020.21:3847.