Proline Analysis Service

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v-innovate Technologies provides an advanced technology platform, including high-performance liquid chromatography/gas chromatography technologies combined with mass-spectrometric technique, to accurately and efficiently analyze and quantify proline content in plants. This high-throughput approach is helpful to further explore the applications of proline in agriculture and plant development.

Overview

There are three forms of proline DL-Proline, L-Proline, and D-Proline. The commonly referred to proline is L-proline, a naturally occurring amino acid. L-Proline is found in almost all proteins and it is abundant in the body, second only to glutamine and alanine. Proline plays an important regulatory role in protein synthesis, metabolism of the body, wound healing, antioxidant and immune response.

Hydroxyproline is found in many plant proteins and is particularly associated with cell wall formation. Plant bodies often have a significant accumulation of proline under a variety of adversities such as drought, high temperature, low temperature, and salinity. In addition, proline has a wide range of applications in clinical, biomaterials, and industrial applications.

GC-MS and LC-MS with high resolution and sensitivity can be used to separate complex sample mixtures for the detection and quantification of specific amino acids. v-innovate Technologies offers an advanced LC-MS technology platform for accurate and efficient analysis and quantification of proline content. In addition, we offer other amino acid and metabolite assays to meet your different needs.

Figure 1. (S)-Proline(left) and (R)-Proline (right) in zwitterionic form at neutral pH

Advantages of Our Prolines Analysis Service

Comprehensive and unified service platform
Experienced sample extraction and data analysis team
MS-based platforms for accurate detection and quantification of prolines
Flexible statistical analysis
High repeatability and low-cost tests

Types of Detectable Substances

DL-Proline

L-Proline

D-Proline

Hydroxyproline

Service Workflow

Figure 2. Proline analysis service workflow.

Quantification methods: external reference method or isotope-labeled internal standard method

Mode: MRM, capable of simultaneously detecting more than 1000 MRM ion pairs

Precision: ≤10^-9g

Positive/Negative polarity switching time: ≤20ms,allowing for the acquisition of Q1/Q3 MRM transition mass spectra in both ionization modes from a single LC-MS/MS run.

Analysis content:

Standard curve drawing
Raw data preprocessing
Absolute quantitation of prolines
Differential metabolites screening
Optimal analyses such as KEGG pathway analysis and hierarchical clustering

Sample Requirements

Plasma ≥ 200 μL/sample
Blood ≥ 200 μL/sample
Animal tissues ≥ 0.2 g/sample
Plant tissues (from leaf, flower, stem, root, or fruit) ≥ 1 g/sample.
Food products
Drugs
Others

We also accept other samples. Our technicians will communicate with you about the specific sample requirements.

Deliverables

Experimental procedure
Instrument parameters
MS raw data files and MS data quality checks
Metabolites quantification data
Custom analysis report

We can provide customers with sample processing, testing, analysis, and other professional technical services, and work with our customers to improve product quality.From sampling to analysis, we provide one-stop services. Our experienced team guarantees to produce credible results at a competitive price.

References

Verbruggen N, Hermans C: Proline accumulation in plants: a review. AMINO ACIDS2008, 35(4):753-759.
Ábrahám E, Rigó G, Székely G, Nagy R, Koncz C, Szabados L: Light-dependent induction of proline biosynthesis by abscisic acid and salt stress is inhibited by brassinosteroid in Arabidopsis. PLANT MOL BIOL2003, 51(3):363-372.

For Research Use Only. Not for use in diagnostic procedures.

Proline Analysis Service