v-innovate Technologies has strong expertise in providing advanced proteomics, metabolomics, glycomics, bioinformatics, and other services for academics and industries in biochemistry, biotechnology, and biopharmaceutical fields, which are powered by the establishment of state-of-the-art mass spectrometry (MS) platforms, coupled with high-performance liquid chromatography (HPLC) or ultra-performance liquid chromatography (UPLC) technology.
Untargeted metabolomics is an analytical approach used for the simultaneous, unbiased analysis of a vast array of compounds that an organism accumulates at a certain point in a given sample, which is used to investigate metabolic phenotypes involving a massive amount of metabolites and elucidate plant-pathogen interactions. Untargeted analyses are especially useful in some cases previous knowledge about the metabolome of the plants or compounds relevant to herbivores is not available. Plant specimens are complex matrices consisting of a myriad of metabolites. Thus, a significant challenge of metabolomics is to identify measured metabolites. Currently, liquid chromatography coupled to mass spectrometry (LC-MS) is the most promising technique for metabolomics in terms of its sensitivity, resolution, precision, throughput, and metabolome coverage. UPLC coupled to high-resolution mass spectrometry (UPLC-MS/MS) is the most commonly used method for untargeted plant metabolomics, which generates a massive amount of data generated. With an integrated set of separation, identification and quantification systems featured with excellent robustness and reproducibility, v-innovate Technologies provides reliable, efficient and cost-effective plant untargeted metabolomics services.
v-innovate Technologies employs state-of-the-art UPLC-MS/MS systems for plant untargeted metabolomics studies. A non-targeted metabolomic analysis generally consists of these steps: sample collection and homogenization, metabolites extraction, raw data acquisition, data processing, and bioinformatics analysis. We have strong expertise in handling a variety of sample types, and ensure the best recovery and unbiased metabolome measurement.
Our primary instruments include:
Fresh plant tissue or seed, weight > 1 g. Samples should be frozen in liquid nitrogen right after collection, and then transferred to -80°C for storage. At least 3 biological replicates.
v-innovate Technologies is proud to offer high-efficient and accurate plant untargeted metabolomics analysis service by applying advanced UPLC- MS/MS systems and techniques. We have extensive experience in the field of plant metabolomics and offer custom service to meet customer's specific research needs, and guarantee comprehensive analysis service with accuracy.
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