Phenylalanine Analysis Service

Overview

Phenylalanine (Phe) is one of the eight essential amino acids in the human body. It cannot be synthesized by the human body and can only be synthesized by plants and microorganisms. The amino acid is metabolized and utilized in the intestinal tract by the cells of the intestinal mucosa and may also be metabolized by the large number and complexity of microflora in the intestine.Phe is an aromatic amino acid. Most of it is oxidized by the body to tyrosine catalyzed by phenylalanine hydroxylase, with which it synthesizes important neurotransmitters and hormones, and participates in the body's glucose and fat metabolism.

There are three different types of phenylalanine: L-phenylalanine, D-phenylalanine and DL-phenylalanine. D-Phenylalanine allows for more effective pain control. L-phenylalanine helps regulate mood, appetite and mental alertness. DL-Phenylalanine can have a positive effect on pain sensitivity and mental state.

Accurate and efficient analysis and quantification of phenylalanine in plants, animals, and products is in demand in the food, pharmaceutical, nutritional, and anti-cancer fields. v-innovate Technologies has an advanced HPLC-MS analytical platform to provide you with phenylalanine detection and content analysis services.

Figure 1. Phenylalanine chemical structure

Applications of Phenylalanine Analysis

• Recognize the role of phenylalanine in plant and animal metabolism
• Determine plant pressure
• Promote animal growth
• Explore the application of phenylalanine in plants and animals

Advantages of Our Phenylalanine Analysis Service

• Comprehensive and unified service platform
• Experienced sample extraction and data analysis team
• MS-based platforms for accurate detection and quantification of phenylalanine
• Flexible statistical analysis
• High repeatability and low-cost tests

Service Workflow

Our newly developed sample preprocessing method provides efficiency, recovery rates consistently exceeding 85%, and environmental protection. Sample preprocessing at v-innovate Technologies complies with in-house standard operation procedures (SOPs), maximizing homogeneity between samples.

Figure 2. Phenylalanine analysis service workflow.

Quantification methods: external reference method or isotope-labeled internal standard method

Mode: MRM, capable of simultaneously detecting more than 1000 MRM ion pairs

Precision: ≤10^-9 g

Positive/Negative polarity switching time: ≤20 ms, allowing for the acquisition of Q1/Q3 MRM transition mass spectra in both ionization modes from a single LC-MS/MS run.

Analysis content:

• Standard curve drawing
• Raw data preprocessing
• Absolute quantitation of phenylalanine
• Differential metabolites screening
• Optimal analyses such as KEGG pathway analysis and hierarchical clustering

Sample Requirements

• Blood/plasma: 500ul/sample
• Urine: 1ml/sample
• Tissue: 200mg/sample
• Cells: 1x10⁷/sample
• Feces: 500mg/sample
• Sample storage and transportation: Store in liquid nitrogen or -80°C, and transport on dry ice.
• Number of biological replicates: At least 6 biological replicates for plant samples, and at least 10 biological replicates for animal samples

Deliverables

• Experimental procedure
• Parameters of liquid chromatography and MS
• Extracted mitochondria and purified protein sample
• Purity analysis report
• MS raw data files and MS data quality checks
• Metabolites quantification data
• Custom analysis report

We can provide customers with sample processing, testing, analysis, and other professional technical services, and work with our customers to improve product quality. From sampling to analysis, we provide one-stop services. Our experienced team guarantees to produce credible results at a competitive price.

References

1. Xueqin Song, Philip L. Lorenzi, et al, Amino Acid Ester Prodrugs of the Anticancer Agent Gemcitabine: Synthesis, Bioconversion, Metabolic Bioevasion, and hPEPT1-Mediated Transport. Analytical Biochemistry, 2019, 2: 157-167.
2. Eiken HG, Knappskog PM, et al, PKU mutation G46S is associated with increased aggregation and degradation of the phenylalanine hydroxylase enzyme. Hum Mutat, 2018, 7: 228-238.