Pelargonidin Analysis Service

v-innovate Technologies' targeted metabolomics services target specific metabolites and pathways of interest, which can accurately monitor dynamic metabolic processes, reveal related metabolic mechanisms and verify potential metabolic biomarkers, so as to guide your research with reliable and accurate measurement results.

• Blank

Remove residual impurities in the instrument, provide a stable detection environment, and monitor the stability of the instrument.

• Mixed standard

Mixed standard mainly monitors the stability of the instrument.

• Random sample repetition

Monitor the stability of the entire extraction process.

Targeted metabolome workflow of pelargonidin

Targeted metabolome studies can establish baseline metabolite levels in tissues or organisms for comparison among experiments under different conditions.

   Design experiments and sample processing

   Preparation of metabolic standards and LC-MS/MS detection

   Make standard curve line

   Extraction of metabolites in samples

   Raw data collection and preprocessing

   Qualitative and quantitative target metabolites

   Bioinformatics analysis (PCA analysis, cluster analysis, difference analysis, etc.)

Sample requirements

Sample type

   If it is a cell sample, centrifuge after sampling, remove the medium, immediately put it in liquid nitrogen, and store it at -80°.

   If it is a tissue sample, please put it in liquid nitrogen immediately after separation and store it at -80°.

Mixed sampling

   Each sample was mixed from more than 6 tissues under the same conditions and quickly put into liquid nitrogen.

   ※The same condition refers to the same period, basically the same phenotype, and the same part.

Sample weight and repeat

   It is recommended that the total amount of fresh and clean plant samples is >1g, and prepare 6 biological replicates.

Storage and transportation

   Dry ice transportation (dry ice volatilizes 3-4 kg /day, please use sufficient dry ice for transportation).

Service process

Project cycle

About 1-3 weeks

v-innovate Technologies has leading technology, first-class R&D and production equipment, and has established a complete metabolome service system. Our professional team has many years of experience in metabolomics, bioinformatics, and statistics, and can help you plan, conduct and report metabolomics research, so that your upstream and downstream experiments can be seamlessly connected. We can provide a wide range of target metabolomics services, including target metabolic pathways, target metabolite analysis, etc. You can also perform personalized customization services at the same time during the data analysis stage. If you have any questions or specific requirements, please feel free to contact us.

References

1. Seo M, Kim H, Lee J H, et al. Pelargonidin ameliorates acetaminophen-induced hepatotoxicity in mice by inhibiting the ROS-induced inflammatory apoptotic response[J]. Biochimie, 2019, 168.
2. Samadder A, Tarafdar D, Das R, et al. Efficacy of nanoencapsulated pelargonidin in ameliorating pesticide toxicity in fish and L6 cells:Modulation of oxidative stress and signalling cascade[J]. Science of The Total Environment, 2019, online first(JUN.25):466-473.
3. Wang L S, Sun X D, Cao Y, et al. Antioxidant and pro-oxidant properties of acylated pelargonidin derivatives extracted from red radish (Raphanus sativus var. niger, Brassicaceae)[J]. Food & Chemical Toxicology An International Journal Published for the British Industrial Biological Research Association, 2010, 48(10):2712-2718.