L-Histidine Analysis Service

v-innovate Technologies provides an advanced technology platform, including high-performance liquid chromatography/gas chromatography technologies combined with mass-spectrometric technique, to accurately and efficiently analyze and quantify proline content in plants and annimals. This high-throughput approach is helpful to further explore the applications of L-histidine in annimal and plant development.

Overview

Histidine (His) is considered an essential amino acid for humans. L-histidine is a semi-essential amino acid, which is especially important for the growth of infants and animals. Mainly found in bananas, grapes, meat, poultry, milk, dairy products and green vegetables. It is often used as a nutritional fortifier and an important component in amino acid infusion and compound amino acid preparations. In the field of medicine, it can be used as biochemical reagents and medicaments for the treatment of heart disease, anemia, rheumatoid arthritis, etc. In animals, L-histidine can improve animal performance, increase muscle carnosine content, and enhance the body’s antioxidant capacity. Although L-histidine plays an important role in human body and animal body weight, it is used in plant cells or animals. The role of different growth conditions in cells is poorly understood. Fortunately, high performance liquid chromatography or gas chromatography can be used to detect and quantify specific amino acids based on their physical and chemical properties. By combining with mass spectrometry, specific amino acids such as L-histidine in different plant or animal samples can be effectively determined.

Figure 1. L-Histidine chemical structure

Applications of L-Histidine Analysis

• Recognize the role of L-histidine in plant and animal metabolism
• Determine plant pressure
• Promote animal growth
• Explore the application of L-histidine in plants and animals

Advantages of Our L-Histidine Analysis Service

Experienced sample extraction and data analysis team

MS-based platforms for accurate detection and quantification of L-histidine

Flexible statistical analysis

High repeatability and low-cost tests

Service Workflow

Our newly developed sample preprocessing method provides efficiency, recovery rates consistently exceeding 85%, and environmental protection. Sample preprocessing at CPR complies with in-house standard operation procedures (SOPs), maximizing homogeneity between samples.

Figure 2. L-Histidine analysis service workflow.

Quantitation method: external reference method or isotope-labeled internal standard method

Mode: MRM, capable of simultaneously detecting more than 1000 MRM ion pairs

Precision: ≤10^-9 g

Positive/Negative polarity switching time: ≤20 ms, allowing for the acquisition of Q1/Q3 MRM transition mass spectra in both ionization modes from a single LC-MS/MS run.

Analysis content:

• Analysis content
• Standard curve drawing
• Raw data preprocessing
• Absolute quantitation of L-histidine
• Differential metabolites screening
• Optimal analyses such as KEGG pathway analysis and hierarchical clustering

Sample Requirements

• Blood/plasma: 500ul/sample
• Urine: 1ml/sample
• Tissue: 200mg/sample
• Cells: 1x10⁷/sample
• Feces: 500mg/sample
• Sample storage and transportation: Store in liquid nitrogen or -80°C, and transport on dry ice.
• Number of biological replicates: At least 6 biological replicates for plant samples, and at least 10 biological replicates for animal samples

Delivery

• Experimental procedure
• Parameters of liquid chromatography and MS
• Extracted mitochondria and purified protein sample
• Purity analysis report
• MS raw data files and MS data quality checks
• Metabolites quantification data
• Custom analysis report

We can provide customers with sample processing, testing, analysis, and other professional technical services, and work with our customers to improve product quality. From sampling to analysis, we provide one-stop services. Our experienced team guarantees to produce credible results at a competitive price.

References

1. Wang Weining, Li Yuanbo, et al, Terahertz spectroscopy study of histidine and arginine. Journal of Physics, 2007.
2. Zhao Xirong, Kang Lianchun, et al, Peutz-Jeghers syndrome fragile histidine triad gene mutation and carcinogenesis.  Cancer: English Version, 2003.
3. Zhang Zhaohui, Zhang Huabin, et al, Preparation and characterization of L-histidine chiral identification imprinting stationary phase. China National Knowledge Infrastructure, 2008.