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Isocitric Acid Analysis Service
Online Inquiryv-innovate Technologies' targeted metabolomics services target specific metabolites and pathways of interest, which can accurately monitor dynamic metabolic processes, reveal related metabolic mechanisms and verify potential metabolic biomarkers, to guide your research with reliable and accurate measurement results.
About Isocitric Acid
Isocitric acid is an isomer of citric acid. Although the amount is small, it is widely present in the biological world. For example, in the leaves of succulent plants such as Bryophyllum or in rubus plants.
Isocitric acid is a component in the tricarboxylic acid cycle.
Isocitric acid is commonly used as a marker to test the authenticity and quality of fruit products (such as citrus juice). For example, in pure orange juice, the ratio of citric acid to D-isocitric acid is usually less than 130. If the isocitrate value is higher than this ratio, it may indicate adulteration of the juice.
Its chemical formula is C6H8O7, and CAS number is 320-77-4.
MS-based technical means can realize the qualitative and quantitative analysis of isocitric acid.
Molecular structure of isocitric acid
Technical Route of Targeted Metabolomics of Isocitric Acid
Sample Requirements
| Sample type |
| Plants, yeasts, microorganisms, etc. Serum, plasma, urine, bile, bile acid, etc. Cells, liver, brain tissue and other animal tissues and feces, etc. There is no restriction on plant varieties. For varieties with special requirements or rare varieties, please contact our staff for more information. |
| Sample demand |
| Plant root / stem / leaf / fruit / seed > 200 mg fresh weight, freeze-dried sample > 0.5 g Cells / microorganisms / cell supernatant / culture medium supernatant: the number of cells or bacteria > 107, supernatant > 2 ml Serum / plasma / urine / ruminal fluid / cerebrospinal fluid / amniotic fluid and other homogeneous liquid samples > 200 µl. (Hemolysis should be avoided. Cerebrospinal fluid can be as low as 100 µl.) Animal tissue / feces related samples > 200mg / sample For other sample types, please consult technical support or sales. |
| Sample mixed and repeat |
| In order to ensure the accuracy of the samples and reduce systematic errors during sampling, it is necessary to select more than 3 materials with the same condition for each sample. ※ The same condition refers to the same period, basically the same phenotype, and the same part. It is recommended that prepare more than 6 biological replicates. |
| Storage and transportation |
| Quick-frozen preservation with liquid nitrogen can minimize the leakage time of plant samples at room temperature, and store it at -80℃. Dry ice transportation (about 3~4 kg dry ice is consumed every day, please use sufficient dry ice for transportation). |
Feedback to Customers
v-innovate Technologies will provide you with detailed technical reports, including
- Experimental steps
- Related mass spectrometry parameters
- Part of the mass spectrum picture
- Raw data
- Metabolic molecular identification results
v-innovate Technologies offers several approaches to metabolomics studies, delivers precise and detailed data and analysis report. We can also customize the methods or establish new methods together with our collaborators, so they are fit-for-purpose and meet your specific needs. If you have any questions or specific requirements, please feel free to contact us.
References
- Kim M J, Lee M Y, Shon J C, et al. Untargeted and targeted metabolomics analyses of blackberries – Understanding postharvest red drupelet disorder. Food Chemistry, 2019, 300:125169.
- Pawlak M, Klupczynska A, Kokot Z J, et al. Extending Metabolomic Studies of Apis mellifera Venom: LC-MS-Based Targeted Analysis of Organic Acids. Toxins, 2019, 12(1):14.
- Wang X, Zhao X, Zhao J, et al. Serum metabolite signatures of epithelial ovarian cancer based on targeted metabolomics. Clinica Chimica Acta, 2021, 518: 59-69.
For Research Use Only. Not for use in diagnostic procedures.
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