Glycolysis Metabolism Service

Glycolysis is a common metabolic pathway in almost all cells. It occurs in the cytoplasm of the cell. Glycolysis consists of ten enzyme-catalyzed reactions whose main purpose is to convert glucose into pyruvate and produce energy in the form of ATP. For cells without mitochondria, like erythrocytes, glycolysis is the only source of energy for ATP. For cells containing mitochondria, glycolysis is the preparatory pathway for the complete oxidation of glucose to produce large amounts of ATP. All cells in the body use glycolysis to produce energy. Some of the intermediates in the glycolytic cycle steps may also have direct biological roles, such as dihydroxyacetone phosphate, which is used in lipid synthesis. By monitoring metabolite levels at key steps of the glycolytic pathway, we can gain useful insights into the overall energy homeostasis of biological systems. Defective glycolysis can lead to various diseases, such as cancer.

To explore in depth the relevance of glycolytic metabolic pathways and related metabolites to the pathophysiology of various diseases affecting human health, v-innovate Technologies offers an integrated and comprehensive service for the analysis of glycolytic pathway key point substances and other metabolites in biological samples.

The Main Applications of Glycolysis Metabolism Service

Biomarker discovery

Disease diagnostics

Drug discovery

Food safety

Metabolic disease research

Figure 1. Glycolysis: the glucose oxidative pathway at cellular levels.

Advantages of Our Glycolysis Metabolism Service

Rich experience in different types of animal sample preparation

State of art facilities

Professional experiment design and data analysis

Competitive price

Short turnaround time

High accuracy, specificity, and sensitivity

Service Workflow

The glycolysis metabolism service provided by v-innovate Technologies is based on our cutting-edge chromatographic separation and mass spectrometry platforms. The experimental workflow involves 4 steps: sample collection, metabolites extraction, MS data analysis and bioinformatics analysis (Figure 2). Our service will be tailored to specific samples and needs for optimal results.

Figure 2. The overall workflow of glycolysis metabolism service.

List of Detectable Glycolysis Metabolites at v-innovate Technologies

Glucose	Glucose 6-phosphate	Fructose 6-phosphate
Fructose 1,6-phosphate	Dihydroxyacetone phosphate	Glyceraldehyde 3-phosphate
1,3-Bisphosphoglyceric Acid	Glyceraldehyde 3-phosphate	2-Phosphoglyceric Acid
Phosphoenolpyruvate Acid	Pyruvate	ATP
ADP

Sample Requirements

We can analyze any biological materials including but not limited to cells and solid tissues from animals. If you need transport your samples to us, please follow the below requirements for different types of sample:

• Blood/plasma: 500ul/sample
• Urine: 1ml/sample
• Tissue: 200mg/sample
• Cells: 1x10⁷/sample
• Feces: 500mg/sample

Shipment condition: dry ice

Report Delivery

• Experimental procedures
• Instrument parameters of liquid chromatography and mass spectrometry
• MS raw data files and the summary of mass spectrometry data quality
• The identification/quantification of lipidic molecules data
• Bioinformatics analysis report (PCA, KEGG, etc.)

Equipped with advanced mass spectrometry for the determination of carbohydrate metabolites, powerful bioinformatic analysis software and a panel of experienced technicians and scientists, we provide customer-tailored targeted glycolysis pathway metabolites analysis services with rapid experimental procedures and easy to read report, to advance your scientific research.

References

1. Arren Bar-Even, Avi Flamholz, Elad Noor and Ron Milo.Rethinking glycolysis: on the biochemical logic of metabolic pathways. Nat Chem Biol. 2012,8:509-517.
2. Robert A Gatenby, Robert J Gillies. Glycolysis in cancer: a potential target for therapy. Int J Biochem Cell Biol. 2007,39:1358-1366.