Glycine Analysis Service

Glycine (Gly) is the structurally simplest amino acid and is involved in a variety of metabolic and pathophysiological processes in the body. v-innovate Technologies can provide efficient and high-quality glycine identification and quantitative analysis using mass spectrometry-based techniques. Advances in gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) techniques have provided new insights into the functional mechanisms of glycine in pharmaceutical, food, agriculture, forestry and animal husbandry research.

Overview

Glycine is associated with the synthesis of proteins and physiologically active molecules related to many important metabolisms. Glycine is involved in signaling as a neurotransmitter in the central nervous system and plays an important role in immunomodulation, cytoprotection, protection against ischemia-reperfusion injury, reduction of free radical production, reduction of shock injury, and prevention of alcoholic liver disease. In terms of biotransformation, glycine is involved in the synthesis of many important nitrogenous substances, such as purine bases, porphyrin rings of heme and cytochromes, creatine, glutathione and glycocholic acid, in addition to constituting proteins. In addition, glycine can also react with a large number of endogenous substances and drugs to affect the metabolic excretion of related drugs and play a detoxifying role.

Glycineis the most commonly used amino acid in plant uptake studies. It is considered as an important source of plant nitrogen source due to its low relative molecular mass, low carbon to nitrogen ratio, and fast diffusion rate in the soil.

In order to better explore the functional role of glycine in life activities, v-innovate Technologies has UPLC-MS/MS analysis system to provide you with glycine content testing service and metabolite analysis during glycine metabolism to accelerate your research progress.

Applications of Glycine Analysis

• Understand the role of glycine in plant cell metabolism
• Regulate the growth and immunity of plants
• Predict the condition of plant protein synthesis
• Improve plant production
• Assess plant quality and phenotyping

Advantages of Our Glycine Analysis Service

High repeatability of qualitative tests

Highly accurate glycine detection

Efficient performance by mass spectrometry-based platforms

Flexible statistical analysis and bioinformatics analysis

Fast turnaround times and affordable prices

Service Workflow

Our newly developed sample preprocessing method provides efficiency, recovery rates consistently exceeding 85%, and environmental protection. Sample preprocessing at v-innovate Technologies complies with in-house standard operation procedures (SOP), maximizing homogeneity between samples.

Figure 1. Glycine analysis service workflow.

Quantification methods: external reference method or isotope-labeled internal standard method

Mode: MRM, capable of simultaneously detecting more than 1000 MRM ion pairs

Precision: ≤10^-9 g

Positive/Negative polarity switching time: ≤20 ms, allowing for the acquisition of Q1/Q3 MRM transition mass spectra in both ionization modes from a single LC-MS/MS run.

Analysis content:

• Standard curve creation
• Raw data preprocessing
• Absolute quantification of Glycine
• Differential metabolites screening
• Optimal analyses such as KEGG pathway analysis and hierarchical clustering

Sample Requirements

• Serum/plasma ≥ 500 μl/sample
• Urine ≥ 1 ml/sample
• Tissue ≥ 200 mg/sample
• Cells: ≥ 1 × 10⁷/sample
• Feces: ≥ 500 mg/sample
• Drugs
• Others

We can also accept other samples. Please contact us for the exact sample size required for the test.

Deliverables

• Experimental procedure
• Parameters of UPLC and MS
• Raw data, chromatograms and mass spectra
• Metabolites quantification data
• Custom analysis report

With rich experience in amino acids analysis, we can provide accurate, efficient, and cost-effective glycine qualitative and quantitative analysis services. The flexibility of our techniques can cope with different samples and provide satisfactory results.

References

1. Schegg KM, Denslow ND, Andersen TT, Bao Y, Cohen SA, Mahrenholz AM, Mann K: Quantitation and identification of proteins by amino acid analysis: ABRF-96AAA collaborative trial. In: Techniques in Protein Chemistry. Edited by Marshak DR, vol. 8: Academic Press; 1997: 207-216.
2. Wang X, Tang D, Huang D: Proteomic analysis of pakchoi leaves and roots under glycine–nitrogen conditions. PLANT PHYSIOL BIOCH 2014, 75:96-104.