Fumaric Acid Analysis Service

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v-innovate Technologies' targeted metabolomics services target specific metabolites and pathways of interest, which can accurately monitor dynamic metabolic processes, reveal related metabolic mechanisms and verify potential metabolic biomarkers, to guide your research with reliable and accurate measurement results.

About Fumaric Acid

Fumaric acid is an organic compound with the formula HO2CCH=CHCO2H. It is a carboxylic acid derived from butene and is a colorless, flammable crystal.

Fumaric acid is used in the production of unsaturated polyester resins, meat product processing, and can also be used as acidity regulators, acidifiers, antioxidant additives, curing accelerators, spices, etc.

Its chemical formula is C4H4O4, and CAS number is 110-17-8.

MS-based technical means can realize the qualitative and quantitative analysis of fumaric acid.

Molecular structure of fumaric acid

Technical Route of Targeted Metabolomics of Fumaric Acid

Sample Requirements

Sample type

Plants, yeasts, microorganisms, etc.
Serum, plasma, urine, bile, bile acid, etc.
Cells, liver, brain tissue and other animal tissues and feces, etc.
There is no restriction on plant varieties. For varieties with special requirements or rare varieties, please contact our staff for more information.

Sample demand

Plant root / stem / leaf / fruit / seed > 200 mg fresh weight, freeze-dried sample > 0.5 g
Cells / microorganisms / cell supernatant / culture medium supernatant: the number of cells or bacteria > 10⁷, supernatant > 2 ml
Serum / plasma / urine / ruminal fluid / cerebrospinal fluid / amniotic fluid and other homogeneous liquid samples > 200 µl.
(Hemolysis should be avoided. Cerebrospinal fluid can be as low as 100 µl.)
Animal tissue / feces related samples > 200mg / sample
For other sample types, please consult technical support or sales.

Sample mixed and repeat

In order to ensure the accuracy of the samples and reduce systematic errors during sampling, it is necessary to select more than 3 materials with the same condition for each sample.
※ The same condition refers to the same period, basically the same phenotype, and the same part.
It is recommended that prepare more than 6 biological replicates.

Storage and transportation

Quick-frozen preservation with liquid nitrogen can minimize the leakage time of plant samples at room temperature, and store it at -80℃.
Dry ice transportation (about 3~4 kg dry ice is consumed every day, please use sufficient dry ice for transportation).

Feedback to Customers

Experimental steps
Related mass spectrometry parameters
Part of the mass spectrum picture
Raw data

Project Cycle

A standard experiment and analysis process takes about 1~4 weeks.

References

Verdonk J C, De Vos C H R, Verhoeven H A, et al. Regulation of floral scent production in petunia revealed by targeted metabolomics. Phytochemistry, 2003, 62(6): 997-1008.
Hatoum D, Annaratone C, Hertog M, et al. Targeted metabolomics study of ‘Braeburn’ apples during long-term storage. Postharvest Biology and Technology, 2014, 96: 33-41.
Wang X, Zhao X, Zhao J, et al. Serum metabolite signatures of epithelial ovarian cancer based on targeted metabolomics. Clinica Chimica Acta, 2021, 518: 59-69.

For Research Use Only. Not for use in diagnostic procedures.

Fumaric Acid Analysis Service