Metabolomics v-innovate Technologies

v-innovate Technologies Metabolomics

Cyanidin Analysis Service

Cyanidin Analysis Service

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v-innovate Technologies' targeted metabolomics services target specific metabolites and pathways of interest, which can accurately monitor dynamic metabolic processes, reveal related metabolic mechanisms and verify potential metabolic biomarkers, so as to guide your research with reliable and accurate measurement results.

About cyanidin

Its IUPAC name is 2-(3,4-Dihydroxyphenyl) chromenylium-3,5,7-triol

Its chemical formula is C15H11O6.

Its CAS number is 13306-05-3.

It is a particular type of anthocyanidin (glycoside version called anthocyanins).

It is a pigment found in many red berries including grapes, bilberry, blackberry, blueberry, cherry, cranberry, elderberry, hawthorn, loganberry, açai berry and raspberry.

Technical features

Instrument platform

  AB Sciex QTRAP® 6500 LC-MS/MS

Three quality control

The molecular structure of cyanidinThe molecular structure of cyanidin

  • Blank

Remove residual impurities in the instrument, provide a stable detection environment, and monitor the stability of the instrument.

  • Mixed standard

Mixed standard mainly monitors the stability of the instrument.

  • Random sample repetition

Monitor the stability of the entire extraction process.

Targeted metabolome workflow of cyanidin

Targeted metabolome studies can establish baseline metabolite levels in tissues or organisms for comparison among experiments under different conditions.

Random sample repetition

Sample requirements

Sample type

  If it is a cell sample, centrifuge after sampling, remove the medium, immediately put it in liquid nitrogen, and store it at -80°.

  If it is a tissue sample, please put it in liquid nitrogen immediately after separation and store it at -80°.

Mixed sampling

  Each sample was mixed from more than 6 tissues under the same conditions and quickly put into liquid nitrogen.

  ※The same condition refers to the same period, basically the same phenotype, and the same part.

Sample weight and repeat

  It is recommended that the total amount of fresh and clean plant samples is >1g, and prepare 6 biological replicates.

Storage and transportation

  Dry ice transportation (dry ice volatilizes 3-4 kg/day, please use sufficient dry ice for transportation).

Service process

Service process

Project cycle

About 1-3 weeks

v-innovate Technologies has leading technology, first-class R&D and production equipment, and has established a complete metabolome service system. Our professional team has many years of experience in metabolomics, bioinformatics, and statistics, and can help you plan, conduct and report metabolomics research, so that your upstream and downstream experiments can be seamlessly connected. We can provide a wide range of target metabolomics services, including target metabolic pathways, target metabolite analysis, etc. You can also perform personalized customization services at the same time during the data analysis stage. If you have any questions or specific requirements, please feel free to contact us.

References

  1. Cooke, D., Schwarz, M., et al., Effect of cyanidin‐3‐glucoside and an anthocyanin mixture from bilberry on adenoma development in the ApcMin mouse model of intestinal carcinogenesis—Relationship with tissue anthocyanin levels[J]. International Journal of Cancer, 2006, 119(9).
  2. Zhang Y, Li Y, et al. Metabolic and molecular analysis of nonuniform anthocyanin pigmentation in tomato fruit under high light[J]. HORTICULTURE RESEARCH, 2019, 6(1).
  3. Elixabet Díaz-de-Cerio, Vito Verardo, et al. Exploratory Characterization of Phenolic Compounds with Demonstrated Anti-Diabetic Activity in Guava Leaves at Different Oxidation States[J].IJMS,2016,17(5).
For Research Use Only. Not for use in diagnostic procedures.

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