Chlorophyll f Analysis Service

Targeted metabolomics is mainly used to identify and quantitatively analyze specific metabolites in samples, this method has been widely used to analyze and compare multiple target metabolites in different physiological states. It is a key analysis method for researches on green plants to improve nutrition and so on. Based on the LC-MS/MS platform, v-innovate Technologies use analytical standard detection methods to perform accurate qualitative and quantitative analysis on the pre-screened and validated or identified potential biomarkers (chlorophyll f).

Sample requirements

Algae

  Fresh algae ≥ 300mg

  Algae freeze-dried powder ≥ 30mg

  And need more than 6 biological replicates

Plant tissue

  Plant samples need to be freeze dried, fresh plant samples need more than 3g. And it is necessary to mix the tissues from 3 plants together as a sample. And need more than 6 biological replicates.

※All samples should be stored at -80°, transported on dry ice, and cannot be repeatedly frozen and thawed.

Workflow of targeted metabolomics of chlorophyll f

Design experiments and sample processing

Standard preparation and mass spectrometry detection

Make standard curve

Extraction of metabolites in samples

Raw data preprocessing

Qualitative and quantitative target metabolites

Bioinformatics analysis (PCA analysis, cluster analysis, difference analysis, etc.)

Analysis of differences between different groups

Extended analysis, such as metabolite screening, KEGG pathway analysis and hierarchical clustering

Provided by v-innovate Technologies

After the project is completed, v-innovate Technologies will provide detailed technical reports, including

  Experimental steps

  Related mass spectrometry parameters

  Mass spectrum picture

  Raw data

  Chlorophyll f content information

Project cycle

About 1-3 weeks

Technical advantages

Qualitatively accurate

  Check the secondary spectra of sample materials and of standard products one by one.

Quantitatively accurate

  Choose the best ion pair to ensure accurate quantification of the substance.

High sensitivity

  Based on advanced mass spectrometry platform to ensure high sensitivity.

Targeted metabolomics based on mass spectrometry can more sensitively and more accurately quantify target metabolites. With decades of experience in mass spectrometry services, v-innovate Technologies undertakes the qualitative and quantitative analysis of a variety of plant metabolites, provides accurate and detailed data and analysis reports. From sample extraction and detection to bioinformatics, v-innovate Technologies can meet your special needs, and has a good record. If you want to know more, please contact us immediately.

References

1. Weihan Song, Qixian Hao, Mengying, et al. Rice OsBT1 regulates seed dormancy through the glycometabolism pathway[J]. Plant Physiology and Biochemistry,2020,151.
2. Jin D, Dai K, Xie Z, et al. Secondary Metabolites Profiled in Cannabis Inflorescences, Leaves, Stem Barks, and Roots for Medicinal Purposes[J]. Scientific Reports, 2020, 10(1).
3. Yu C, Zhao X, Qi G, et al. Integrated analysis of transcriptome and metabolites reveals an essential role of metabolic flux in starch accumulation under nitrogen starvation in duckweed[J]. Biotechnology for Biofuels, 2017, 10(1).