Benzoic Acid Analysis Service

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v-innovate Technologies' targeted metabolomics services target specific metabolites and pathways of interest, which can accurately monitor dynamic metabolic processes, reveal related metabolic mechanisms and verify potential metabolic biomarkers, to guide your research with reliable and accurate measurement results.

About Benzoic Acid

Benzoic acid is an aromatic acid organic compound and the simplest aromatic acid. It was originally made from benzoin gum, so it is called benzoic acid.

Benzoic acid is widely present in nature in the form of free acid, ester or its derivatives.

It is mainly used for the preparation of sodium benzoate preservative, and for the synthesis of medicines and dyes, and also for the preparation of plasticizers, mordants, fungicides and perfumes.

It can be prepared by direct oxidation of toluene in the presence of manganese dioxide, or by decarboxylation of phthalic anhydride with water vapor.

Its chemical formula is C7H6O2, and CAS number is 65-85-0.

MS-based technical means can realize the qualitative and quantitative analysis of benzoic acid.

Molecular structure of benzoic acid

Technical Route of Targeted Metabolomics of Benzoic Acid

Sample Requirements

Sample type

Plants, yeasts, microorganisms, etc.
Serum, plasma, urine, bile, bile acid, etc.
Cells, liver, brain tissue and other animal tissues and feces, etc.
There is no restriction on plant varieties. For varieties with special requirements or rare varieties, please contact our staff for more information.

Sample demand

Plant root / stem / leaf / fruit / seed > 200 mg fresh weight, freeze-dried sample > 0.5 g
Cells / microorganisms / cell supernatant / culture medium supernatant: the number of cells or bacteria > 10⁷, supernatant > 2 ml
Serum / plasma / urine / ruminal fluid / cerebrospinal fluid / amniotic fluid and other homogeneous liquid samples > 200 µl.
(Hemolysis should be avoided. Cerebrospinal fluid can be as low as 100 µl.)
Animal tissue / feces related samples > 200mg / sample
For other sample types, please consult technical support or sales.

Sample mixed and repeat

In order to ensure the accuracy of the samples and reduce systematic errors during sampling, it is necessary to select more than 3 materials with the same condition for each sample.
※ The same condition refers to the same period, basically the same phenotype, and the same part.
It is recommended that prepare more than 6 biological replicates.

Storage and transportation

Quick-frozen preservation with liquid nitrogen can minimize the leakage time of plant samples at room temperature, and store it at -80℃.
Dry ice transportation (about 3~4 kg dry ice is consumed every day, please use sufficient dry ice for transportation).

Feedback to Customers

Experimental steps
Related mass spectrometry parameters
Part of the mass spectrum picture
Raw data

Project Cycle

A standard experiment and analysis process takes about 1~4 weeks.

References

Verdonk J C, De Vos C H R, Verhoeven H A, et al. Regulation of floral scent production in petunia revealed by targeted metabolomics. Phytochemistry, 2003, 62(6): 997-1008.
Hatoum D, Annaratone C, Hertog M, et al. Targeted metabolomics study of ‘Braeburn’ apples during long-term storage. Postharvest Biology and Technology, 2014, 96: 33-41.
Wang X, Zhao X, Zhao J, et al. Serum metabolite signatures of epithelial ovarian cancer based on targeted metabolomics. Clinica Chimica Acta, 2021, 518: 59-69.

For Research Use Only. Not for use in diagnostic procedures.

Benzoic Acid Analysis Service