Baicalin Analysis Service

v-innovate Technologies provides high performance liquid chromatography with diode array detection and mass spectrometer (HPLC-DAD-MS) based methods for baicalin analysis, which provides sufficient stability, sensitivity and high resolution for identification and quantification of baicalin. This approach has the advantages of simple, stable, fast, accurate, specific and reproducible, which can help researchers to analyze baicalin effectively.

Overview

Baicalin is a kind of flavonoid extracted and isolated from the dried root of scutellaria baicalensis georgi. Baicalin dissolves in alkali and ammonia and turns yellow at first, then turns black and brown soon. Baicalin has the effects of anti-tumor, anti-pathogen and anti-allergic reaction, and has a protective effect on liver injury, the improvement of diabetic nephropathy, the repair and protection of brain injury, and also has a certain effect on retinopathy. It can be used for bacteriostasis, diuresis, anti-inflammatory, cholesterol-lowering, anti-thrombosis, easing asthma, detoxification, hemostasis and anti-allergic reaction. Baicalin has a wide range of uses, and it is very important to establish an analytical method for baicalin in plant metabolites.

v-innovate Technologies provides the simple, rapid, accurate, affordable and highly automated analytical method and equipment to analyse baicalin. High performance liquid chromatography with diode array detection and mass spectrometer (HPLC-DAD-MS) can solve some of the problems associated with baicalein analysis. Improved extraction and purification steps can also increase the sensitivity of the analytical method.

Applications of Baicalin Analysis

• Qualitative analysis of the main types of baicalin
• Quantitative analysis of baicalin content
• Ensure safety and quality
• Provide basis for improvement

Advantages of Our Baicalin Analysis Service

• Focus on innovation.
• International leading talent team.
• Build a community of interests with customers.
• To solve professional problems with professional skills.

Service Workflow

Our newly developed sample preprocessing method provides safety, environmental protection, rapidness, effectiveness, and the average recovery rate is over 99%. Sample preprocessing at v-innovate Technologies complies with the in-house standard operation procedures (SOP), ensuring maximized homogeneity between samples.

Figure 1. Baicalin analysis service workflow.

Mass spectrometric conditions: electrospray ion source positive ion mode, multi-reaction monitoring mode data collection

Mobile phase: formic acid - water - acetonitrile

Linear range: r>0.99

Elution time: dozens of minutes, slightly different according to different ingredients

Analysis content:

• Standard curve creation
• Raw data preprocessing
• Absolute quantitation of baicalin
• Differential metabolites screening
• Optimal analyses

Sample Requirements

1. Fresh tissues: >1 g. If possible, samples should be frozen in liquid nitrogen right after collection, and then transferred to -80°C for storage.

2. Product samples.

At least 3 biological replicates.

Delivery

• Experimental procedure
• Parameters of HPLC-DAD-MS
• Purity analysis report
• HPLC-DAD-MS raw data files and HPLC-DAD-MS data quality checks
• Metabolites quantification data
• Custom analysis report

HPLC-DAD-MS based profiling of baicalin enables quantitative analyses in a faster, more convenient, and more sensitive manner. With decades of experience in HPLC-DAD-MS services, v-innovate Technologies has a proven track record supporting diverse baicalin detection and quantitation. We can meet your specific project requirements, from sample to bioinformatics.