Arecoline Analysis Service

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Arecoline is the most important alkaloid in the chemical composition of betel nut, with certain pharmacological effects and toxic side effects. Based on an advanced HPLC-MS platform, v-innovate Technologies provides arecoline targeting analysis services to help analyze dynamic metabolic processes and reveal relevant metabolic mechanisms, thus guiding your research with reliable and accurate measurement results.

About Arecoline

Arecoline is an M agonist that excites cholinergic M receptors (muscarinic receptors) and stimulates sympathetic nerves. Arecoline acts on the gastrointestinal digestive system, mainly on the gastrointestinal propulsion, and also promotes small intestine peristalsis. Arecoline protects against Ox-LDL-induced vascular endothelial damage and inhibits the overexpression of high-fat (or high-sugar)-induced vascular endothelial cell adhesion molecules. However, arecoline also has toxic side effects, and the most studied and concentrated effect is on oral cells. Studies have shown that chewing rough betel nut can cause oral mucosal inflammation, oral submucosal fibrosis, DNA damage and malignancy.

In order to better understand the functions and mechanisms of arecoline, and to promote the application of arecoline in anthelmintic, bactericidal, anti-thrombotic, anti-atherosclerotic, oral and neurological applications, it is necessary to identify and analyze the changes in arecoline content using precise and reliable techniques.

Molecular structure of arecoline

Sample Requirements

Tissue ≥ 200 mg/sample
Plasma ≥ 200 μL/sample
Blood ≥ 500 μL/sample
Cells or bacteria > 107, supernatant > 2 ml
Drugs ≥ 0.2 g/sample
For other sample types, please consult technical support or sales.

In order to ensure the accuracy of the samples and reduce systematic errors during sampling, it is necessary to select more than 3 materials with the same condition for each sample. The same condition refers to the same period, basically the same phenotype, and the same part. It is recommended that prepare more than 6 biological replicates.

Workflow of Targeted Metabolomics Service of Arecoline

Targeted metabolomics is a study that identifies and quantitatively analyzes known metabolites in a sample. Its advantages are high specificity and accuracy. Therefore, this method has been widely used to analyze and compare multiple target metabolites in different physiological states.

Workflow of targeted metabolomics service of arecoline

Delivery Standard

Complete experimental report, including methods, instrument parameters, reagents, consumables information and data analysis results of the process, etc. (Chinese version and English version, or one of them)

Complete lab report (including information on methods, instrument parameters, reagents, and consumables)
Raw data
Mass spectrum picture (section)
Metabolic molecular identification results

Turnaround time: About 1-4 weeks.

v-innovate Technologies has leading technology, first-class R&D and production equipment. Our professional team has many years of experience in metabolomics, bioinformatics, and statistics, and can help you plan, conduct and report metabolomics research, so that your upstream and downstream experiments can be seamlessly connected. We can provide a wide range of target metabolomics services, including target metabolic pathways, target metabolite analysis, etc. You can also perform personalized customization services at the same time during the data analysis stage. If you have any questions or specific requirements, please feel free to contact us.

References

Wu J, Cui C, Zhang H, et al. Identifying new compounds with potential pharmaceutical and physiological activity in Areca catechu and Areca triandra via a non‐targeted metabolomic approach[J]. Phytochemical Analysis, 2021.
Yao Y, Wang G B, Man S L, et al. Toxicological advances of traditional medicine in 2019[J]. Traditional Medical Research (TMR), 2020, 005(002):P.83-89.
Luca V D, Salim V, Atsumi S M, et al. Mining the Biodiversity of Plants: A Revolution in the Making[J].Science, 2012.

For Research Use Only. Not for use in diagnostic procedures.

Arecoline Analysis Service